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1. USING IMAGEJ SOFTWARE FLUORESCENCE MANUAL
2. USING IMAGEJ SOFTWARE FLUORESCENCE FULL
3. USING IMAGEJ SOFTWARE FLUORESCENCE SERIES

IĪm grateful to the unacknowledged authors, and hope that they are not offended if The authorship of the original macros was often difficult to determine. I have adopted and adapted a number of macros contributed by various NIH Image

USING IMAGEJ SOFTWARE FLUORESCENCE MANUAL

Manual is dedicated to those many loyal supporters of all of us at NIH, NSF, DOD, Who work so hard to establish those programs that provide the necessary funds. To obtain grant support, we often neglect to sufficiently thank the many dedicated scientists Human Brain Project and the Brain Database Project. This manual was prepared with the direct support of NIMH and the The work that led to this manual was supported by grants to H. With many thanks to Wayne Rasband for his invaluable contributions to scientists ofĪll disciplines, and his advancement of image processing. Stack Modifications, Z-Projections, Stereo Pairs Operations on Confocal Files: Import, Split, Merge List of Macros provided in Confocal Macros ADDENDA TO NIH IMAGE CONFOCAL IMAGING MANUAL USE OF NIH IMAGE FOR IMAGE COLLECTION AND INSTRUMENT CONTROL PRINTING ON VIDEO PRINTER, DYE SUBLIMATION PRINTER, SLIDE MAKER AND VCR I) Exporting Stacks To QuickTime Movies And VCR Recordings Of Stack Color Stereo Images Of A Double Labeled Section Stereo Pair Of Single Labeled Section In Colorģ.

USING IMAGEJ SOFTWARE FLUORESCENCE SERIES

H) Make A Stereo Pair Or Series Of Stereos?Ģ. G) Animating A 3D-Series (Producing "Apparent Rotation") Optimizing Settings For Project Function Of Z-Seriesĭ) Reslicing The Z-Series Along Alternate Planes: (X-Z, Y-Z And Theta-Z)Į) Reslicing To Make A Z-Series In An Alternate Planeį) Generating A 3D-Series From A Z-Series Projection Of A Z-Series And 3D RotationsĪ) Stepping Through A Z-Series Using StacksĬ) Projecting A Z-Series Onto A Single PlaneĢ.

Merging Pairs Of Double Labeled Sectionsī) Double Labeled Sections: Building A Stack (Best Done Using A Macro)Ĭ) Compare Results Of NIH Image 8-Bit Merge With Adobe Photoshop 24-Bit Mergeĭ) Adjusting Color Contrast On Sections In A Stack (See Macros)Į) Merging A Double Labeled Pair Of Z-Series Using A MacroĢ. Caution On Scale And Rotation Of Imagesī) Linear And Non-Linear LUTs, Including Custom LUTsĬ) Enhance Contrast Operator In NIH Imageġ.

USING IMAGEJ SOFTWARE FLUORESCENCE FULL

Evaluating The Quality Of Your Original CLSM ImageĪ) Making Sure That Your Original CLSM Image Uses The Full Range Of 8-Bit Values (0-255)Ī) Cropping Images, Erasing, Superimposing Text, Scale Bars, Rotating And Scaling ImagesĢ. A Few Words Of Caution About Using Zip DisksĮ) Magneto-Optical Disks For Archival Storageġ. Transferring Files From A DOS, DOS/Windows Or OS/2 Based Computer To A Macintoshġ. On-Chip Integration, Multiple Labeled Sections, And RGB Images Practical Guidelines For Implementing On-Chip IntegrationĨ. Fluorescent Excitation/Emission: A Moving TargetĦ. Evaluation of Fluorescent Images Prior to Confocal MicroscopyĢ. EVALUATING FLUORESCENT IMAGES WITH NIH IMAGE PRIOR TO CONFOCAL IMAGINGġ. Cataloging Software: Where Are The Files When You Need Them? Other Software Used In Conjunction With NIH Imageħ. Loading NIH Image And The Confocal Macrosĥ. NIH Image On Macintosh And PC/Windows 95Ĥ. : Use In Fluorescence and Confocal Microscopyġ. Fluorescence and Confocal Microscopy NIH Image